Translations:Champignon Inoculation d'une culture liquide/64/en

• Follow step 1 of this tutorial.
• Boil water in your pan.
• Take your previously prepared jar (see step 2), fill it with boiled water and unrefined beet sugar (volume 4% of the volume of water). Close your jar, cover it with aluminum foil, emphasizing the opening area of ​​the lid.
• Fill your pot with water again and boil it.
• Place your wrapped jar in your saucepan and leave it covered for 2 hours (sterilization).
• In your kitchen, turn on your hood and clean your worktop with alcohol.
• Place tealights all around your work area.
• Light the candles and take your syringe of liquid mycelium out of the refrigerator, clean (alcohol 70°) and place it in the center of the work area.
• Take your syringe and needle out of their bag. Insert the needle.
• Take your jar of liquid culture, clean the outside of this jar with 70° alcohol and place it in your work area.
• Take two sterile compresses and soak them in alcohol.
• Place the needle of your syringe on the flame of one of the candles and wait for the tip to turn red.
• Then place it between the two compresses soaked in alcohol.
• Completely empty the mycelium from your syringe into your jar.
• Wrap your jar with aluminum foil, fabric or paper
• Place it in an environment at 20°C and wait about two weeks. You can shake your jar so that your mycelium spreads even more and take the opportunity to see its evolution

Watch out for the light!